Van der Schueren B, Cassiman J J, Van den Berghe H
J Cell Sci. 1977 Aug;26:101-17. doi: 10.1242/jcs.26.1.101.
Twenty-four-hour-old aggregates of human diploid skin fibroblasts are allowed to attach to a glass or plastic substratum. As a result of this attachment the cells in the aggregate demonstrate rapid and generalized changes in cell shape, cell surface and cytoplasm ultrastructure and in their ability to incorporate [3H]thymidine. Within 24 h they grow out on the substratum to attain the regular monolayer configuration. During the process of leaving the aggregate for the substratum a great number of different morphogenetic properties are displayed by the cells, resembling the properties of embryonic or epithelial cells. The simultaneous occurrence of this great variety of cell shape and cell surface changes, many of them unusual for fibroblasts, as well as the concurrent formation of organized cytoplasmic structures - microfilaments, microtubules - at localized areas of the cells, makes this system a potentially useful tool in the study of cell behaviour.
将24小时龄的人二倍体皮肤成纤维细胞聚集体置于玻璃或塑料基质上使其附着。由于这种附着，聚集体中的细胞在细胞形状、细胞表面和细胞质超微结构以及掺入[3H]胸腺嘧啶核苷的能力方面表现出快速而广泛的变化。在24小时内，它们在基质上生长并形成规则的单层结构。在离开聚集体附着到基质的过程中，细胞表现出许多不同的形态发生特性，类似于胚胎细胞或上皮细胞的特性。同时出现如此多种细胞形状和细胞表面变化，其中许多对成纤维细胞来说是不寻常的，以及在细胞局部区域同时形成有组织的细胞质结构——微丝、微管，使得这个系统成为研究细胞行为的一个潜在有用工具。
