Savage D D, Mendels J, Frazer A
J Pharmacol Exp Ther. 1980 Feb;212(2):259-63.
Rats were administered either monoamine oxidase inhibitors or serotonin uptake inhibitors for either 1, 4 or 16 days. The binding of [3H]serotonin to brain homogenates and the concentration of serotonin in brain was measured at these times. Treatment with inhibitors of serotonin uptake did not change the specific binding of [3H]serotonin in either cerebral cortex or hippocampus, nor did it produce any consistent alterations in the concentration of serotonin in the cerebral cortex. In contrast, monoamine oxidase inhibitors capable of inhibiting A-type monoamine oxidase significantly decreased [3H]serotonin binding after both 4 and 16 days of treatment; serotonin concentrations were significantly elevated at all time intervals. Inhibitors of B-type monoamine oxidase had no effect on either [3H]serotonin binding or serotonin concentrations in cerebral cortex. The reduction in labeled serotonin binding caused by monoamine oxidase inhibitors is due to a decrease in the maximum number of specific binding sites with no change in the affinity of the binding sites for labeled serotonin.
给大鼠分别服用单胺氧化酶抑制剂或血清素摄取抑制剂1天、4天或16天。在这些时间点测量[3H]血清素与脑匀浆的结合以及脑中血清素的浓度。血清素摄取抑制剂处理并未改变[3H]血清素在大脑皮层或海马体中的特异性结合，也未对大脑皮层中血清素的浓度产生任何一致的改变。相比之下，能够抑制A型单胺氧化酶的单胺氧化酶抑制剂在处理4天和16天后显著降低了[3H]血清素结合；在所有时间间隔内血清素浓度均显著升高。B型单胺氧化酶抑制剂对大脑皮层中的[3H]血清素结合或血清素浓度均无影响。单胺氧化酶抑制剂引起的标记血清素结合减少是由于特异性结合位点的最大数量减少，而结合位点对标记血清素的亲和力没有变化。
