Krell R D, Chakrin L W
Int Arch Allergy Appl Immunol. 1978;56(1):39-47. doi: 10.1159/000232001.
The ascaris antigen-induced release of histamine and a slow-reacting substance of anaphylaxis (SRS-A) from passively sensitized fragmented canine lung is further characterized. Histamine and SRS-A were released within 30 sec of antigen challenge, reached a maximum at 7 and 10 min, respectively, and thereafter appeared to remain constant to 30 min. Contractions of guinea pig ileum produced by canine SRS-A were competitively antagonized by the SRS-A antagonist FPL-55712. Indomethacin and deuterium oxide enhanced antigen-induced SRS-A release from canine lung but had little effect on histamine release. The ability of several chemically novel 'antiallergic agents' to inhibit mediator release was evaluated. Inhibition of histamine release, and to a lesser extent SRS-A release, by one of these compounds was shown to vary with time and temperature. It is concluded that fragmented canine lung, while disclosing some qualitative pharmacological differences from other species, is a useful in vitro model of immediate hypersensitivity reactions.
对犬蛔虫抗原诱导被动致敏的犬肺碎片释放组胺和过敏反应慢反应物质（SRS - A）的现象进行了进一步研究。组胺和SRS - A在抗原攻击后30秒内释放，分别在7分钟和10分钟时达到峰值，此后至30分钟似乎保持稳定。犬SRS - A引起的豚鼠回肠收缩被SRS - A拮抗剂FPL - 55712竞争性拮抗。吲哚美辛和氧化氘增强了抗原诱导的犬肺SRS - A释放，但对组胺释放影响很小。评估了几种化学结构新颖的“抗过敏剂”抑制介质释放的能力。其中一种化合物对组胺释放的抑制作用，以及对SRS - A释放较小程度的抑制作用，显示出随时间和温度而变化。得出的结论是，犬肺碎片虽然与其他物种存在一些定性的药理学差异，但仍是速发型过敏反应有用的体外模型。
