Hansen S L, Mazer F D, Klinefelter H F
Am J Clin Pathol. 1980 Jan;73(1):110-3. doi: 10.1093/ajcp/73.1.110.
The Macro-Vue Card Test for Rheumatoid Factor (Hynson, Westcott, and Dunning) is a new commercially available card agglutination test designed for qualitative and quantitative procedures. Coded sera from 195 patients, six known positive sera, and two positive serum pools were tested using four commercial kits. The commercial kits evaluated included latex (Hyland RA Test, Behring-Rapi/tex RF Test), sheep erythrocytes (Wampole, Rheumaton), and charcoal (HWD-Macro-Vue RF Card Test) as carrier particles. Using the prescribed quantitative procedures, the percentages of positive and negative results for rheumatoid factor detected by each product were determined after the (serum) code was broken. The Macro-Vue test compares well with the Behring and Hyland latex dilution systems, and appears to detect more positive sera than the Wampole test. Titers obtained using the Macro-Vue test were more reproducible day to day with known positive sera than were titers obtained using any of the other kits.
类风湿因子宏视卡片试验(海森、韦斯科特和邓宁公司产品)是一种新的市售卡片凝集试验,可用于定性和定量检测。使用四种商用试剂盒对195例患者的编码血清、六份已知阳性血清和两份阳性血清混合样本进行了检测。所评估的商用试剂盒包括以乳胶(海兰类风湿因子试验、贝林-拉皮泰克斯类风湿因子试验)、绵羊红细胞(万波勒公司的风湿因子试剂、 Rheumaton)和木炭(海森-韦斯科特-邓宁类风湿因子宏视卡片试验)作为载体颗粒。按照规定的定量检测程序,在血清编码破解后,确定每种产品检测出的类风湿因子阳性和阴性结果的百分比。宏视试验与贝林和海兰乳胶稀释系统相比效果良好,并且似乎比万波勒试验能检测出更多的阳性血清。对于已知阳性血清,使用宏视试验获得的滴度在不同日期之间的重复性比使用其他任何试剂盒获得的滴度都要好。