Johnson J E, Crawford S, Jorgensen J H
J Clin Microbiol. 1982 Aug;16(2):299-302. doi: 10.1128/jcm.16.2.299-302.1982.
The Macro-Vue Card Test (Hynson, Wescott, and Dunning, Baltimore, Md.) for rapid quantitation of gentamicin in serum was compared with bioassay and radioimmunoassay procedures on sera from 100 patients. Regression analysis of paired results from the bioassay (using Klebsiella pneumoniae ATCC 27799) and the Macro-Vue Card Test indicated a correlation coefficient of 0.89, whereas comparing radioimmunoassay and Card Test results yielded a correlation coefficient of 0.83. The bioassay-radioimmunoassay correlation coefficient was 0.88. The data were further examined by grouping the sera of the patients into three categories based on therapeutic concentration ranges as follows: less than 2 micrograms/ml, subtherapeutic range; 2 to 8 micrograms/ml, therapeutic range; greater than 8 micrograms/ml, potentially toxic range. Of 100 specimen values, 81 fell into the same concentration range when bioassay and Card Test values were compared. Of the 19 disagreements, 12 were considered minor. Of 100 specimen values compared by radioimmunoassay and the Card Test, 88 fell into the same concentration range. Of the 12 disagreements, 6 were considered minor. Therefore, the Macro-Vue Card Test for gentamicin compared favorably with both the bioassay and radioimmunoassay procedures. The minimal time required to perform the Card Test (12 to 15 min) makes it attractive for situations in which immediate results are needed.
采用Macro-Vue卡片检测法（由位于马里兰州巴尔的摩市的Hynson、Wescott和Dunning公司生产）对100例患者血清中的庆大霉素进行快速定量，并与生物测定法和放射免疫测定法进行比较。对生物测定法（使用肺炎克雷伯菌ATCC 27799）和Macro-Vue卡片检测法的配对结果进行回归分析，结果显示相关系数为0.89，而放射免疫测定法与卡片检测法结果的相关系数为0.83。生物测定法与放射免疫测定法的相关系数为0.88。根据治疗浓度范围将患者血清分为三类，对数据作进一步分析：低于2微克/毫升为治疗不足范围；2至8微克/毫升为治疗范围；高于8微克/毫升为潜在中毒范围。比较生物测定法与卡片检测法的100个样本值，有81个落入相同浓度范围。在19个不一致结果中，12个被认为差异较小。比较放射免疫测定法与卡片检测法的100个样本值，有88个落入相同浓度范围。在12个不一致结果中，6个被认为差异较小。因此，用于检测庆大霉素的Macro-Vue卡片检测法与生物测定法和放射免疫测定法相比都具有优势。进行卡片检测所需的最短时间（12至15分钟）使其在需要即时结果的情况下颇具吸引力。
