Bailey D L, Lowe R E, Dame D A, Seawright J A
Am J Trop Med Hyg. 1980 Jan;29(1):141-9. doi: 10.4269/ajtmh.1980.29.141.
Technology was developed for mass rearing males of the genetically altered MACHO strain of Anopheles albimanus Wiedemann, which allowed for elimination of females by treating the eggs with propoxur (o-isopropoxyphenyl methylcarbamate). This made it possible to eliminate virtually all the females (potential malaria vectors) being released in the field, and also reduced the space previously devoted to larval rearing by 50%, since the females were eliminated in the egg stage. Also, the difficulties involved in separating the sexes with previous techniques were eliminated. Because there is some genetic recombination, about 0.2% of the MACHO males become susceptible and an equal number of females become resistant each generation. Thus after 6-12 months, the strain is purged to remove these contaminants. With this system an average of more than 1 million pupae per day was produced during 3 weeks of a 5-week period, and an average of 968.2 thousand per day during the entire period. The pupae produced were 99.9% males with an average adult emergence of 90%.
已研发出用于大规模饲养经基因改造的白纹伊蚊MACHO品系雄性蚊子的技术，该技术通过用残杀威（邻异丙氧基苯基甲基氨基甲酸酯）处理蚊卵来消除雌性蚊子。这使得几乎所有释放到野外的雌性蚊子（潜在的疟疾传播媒介）得以消除，并且由于雌性蚊子在卵期就被消除，之前用于幼虫饲养的空间也减少了50%。此外，以往技术在区分雌雄方面所涉及的困难也得以消除。由于存在一些基因重组现象，每一代约有0.2%的MACHO雄性蚊子变得易感，同时有等量的雌性蚊子变得具有抗性。因此，在6至12个月后，该品系会被净化以去除这些污染物。在为期5周的时间段内，有3周时间平均每天生产超过100万个蛹，在整个期间平均每天生产96.82万个蛹。所生产的蛹中有99.9%为雄性，平均成虫羽化率为90%。
