Hong K, Trudell J R, O'Neil J R, Cohen E N
Anesthesiology. 1980 Jan;52(1):16-9. doi: 10.1097/00000542-198001000-00004.
Nitrous oxide labeled with a stable heavy nitrogen isotope was used for in-vitro studies of nitrous oxide metabolism in man and rat. At 5 per cent oxygen tension, which is comparable to normal oxygen tension in the intestine in vivo, each gram of intestinal contents during a 16-hr in-vitro incubation produced 47 +/- 13 nmol of molecular nitrogen for the rat and 103 +/- 17 nmol for man. Active reductive metabolism of nitrous oxide by intestinal contents was significantly inhibited by antibiotics and by 20 per cent oxygen tension. It is suggested that the reduction of nitrous oxide to nitrogen may proceed through a single-electron transfer process with formation of free radicals. Under these circumstances, metabolism of nitrous oxide could produce toxic intermediates, even thought the end-metabolite is inert.
用稳定重氮同位素标记的氧化亚氮用于人体和大鼠氧化亚氮代谢的体外研究。在5%的氧分压下（这与体内肠道中的正常氧分压相当），在16小时的体外培养过程中，每克大鼠肠道内容物产生47±13纳摩尔分子氮，人体肠道内容物产生103±17纳摩尔分子氮。肠道内容物对氧化亚氮的活性还原代谢受到抗生素和20%氧分压的显著抑制。有人提出，氧化亚氮还原为氮可能通过单电子转移过程进行，同时形成自由基。在这种情况下，即使最终代谢产物是惰性的，氧化亚氮的代谢也可能产生有毒中间体。
