全血中锌原卟啉IX、原卟啉IX和粪卟啉的液相色谱同时测定法
Simultaneous liquid-chromatographic determination of zinc protoporphyrin IX, protoporphyrin IX, and coproporphyrin in whole blood.
作者信息
Gotelli G R, Wall J H, Kabra P M, Marton L J
出版信息
Clin Chem. 1980 Feb;26(2):205-8.
PMID:7353266
Abstract
We describe a method for simultaneously measuring concentrations of coproporphyrin, zinc protoporphyrin IX, and protoporphyrin IX in whole blood by liquid chromatography, with use of reversed-phase ion-pair system, fluorometric detection, and internal standardization. Each analysis requires 10 microL of whole blood and 15 min total analysis time. Analytical recovery ranged from 84 to 92%, day-to-day precision (CV) from 5 to 12%. Uroporphyrin, though not studied in detail, can also be detected by this method.
摘要
我们描述了一种通过液相色谱法同时测量全血中粪卟啉、锌原卟啉IX和原卟啉IX浓度的方法，该方法采用反相离子对系统、荧光检测和内标法。每次分析需要10微升全血，总分析时间为15分钟。分析回收率在84%至92%之间，日间精密度（CV）在5%至12%之间。尿卟啉虽然未进行详细研究，但也可通过该方法检测。
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