Department of Chemistry and Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, SiMing Street, Xiamen 361005, China.
Talanta. 2010 Sep 15;82(4):1516-20. doi: 10.1016/j.talanta.2010.07.034. Epub 2010 Jul 23.
Fluorescence spectroscopy provides high sensitivity in quantitative analysis. However, due to spectral interference, it is difficult to determine the individual components of fluorescent multi-component mixtures in such complicated and important body matrices as blood, urine and feces without any pre-separation. In this study, a simple and rapid approach based on non-linear variable-angle synchronous fluorescence spectrometry coupled with partial least squares analysis (NLVASF/PLS) was developed for the simultaneous determination of protoporphyrin IX (PP), uroporphyrin III (UP) and coproporphyrin III (CP). The detection limits were 0.18, 0.29 and 0.24 nmol L(-1) for protoporphyrin IX (PP), uroporphyrin III (UP) and coproporphyrin III (CP), respectively. The individual components of blood porphyrins were quantified, by this method, simultaneously in one scan with only about 30s. The recoveries of this method were above 80% in human whole blood samples. This method provided a potential tool for the determination of porphyrins in whole blood and the differential diagnosis of porphyria, especially for rapid routine screening of large number of samples.
荧光光谱法在定量分析中具有很高的灵敏度。然而，由于光谱干扰，在没有任何预分离的情况下，很难确定血液、尿液和粪便等复杂而重要的生物基质中荧光多组分混合物的各个成分。在这项研究中，开发了一种基于非线性变角同步荧光光谱法结合偏最小二乘法分析（NLVASF/PLS）的简单快速方法，用于同时测定原卟啉 IX（PP）、尿卟啉 III（UP）和粪卟啉 III（CP）。原卟啉 IX（PP）、尿卟啉 III（UP）和粪卟啉 III（CP）的检测限分别为 0.18、0.29 和 0.24 nmol L(-1)。该方法仅需约 30 秒即可在一次扫描中同时定量测定血液卟啉的各个成分。该方法在人全血样品中的回收率均高于 80%。该方法为全血中卟啉的测定和卟啉病的鉴别诊断提供了一种潜在的工具，特别是用于大量样品的快速常规筛选。
