Hurst R E, Settine J M, Floyd W M, Lorincz A E
Clin Chim Acta. 1980 Jan 31;100(3):307-11. doi: 10.1016/0009-8981(80)90280-6.
This study was carried out in order to determine whether osteogenesis imperfecta affected individuals showed a different pattern of glycosaminoglycan excretion in urine than do normals. Quantitative excretion was compared by three different methods, and the qualitative composition of the excreted glycosaminoglycan was compared by electrophoresis. No difference was noted in the amount of glycosaminoglycan excreted by normal or affected individuals as measured by macromolecular uronic acid, Alcian Blue bound by urinary constituents or amount of glycosaminoglycan actually isolated by partition. However, the affected individuals showed a statistically significant increase in Alcian Blue binding over macromolecular uronic acid not seen in the normal group, which may possibly indicate the excretion of a substance capable of binding Alcian Blue, but which is not glycosaminoglycan, by osteogenesis imperfecta affected individuals. No qualitative differences in glycosaminoglycan excretion between the two groups were detectable by electrophoresis.
开展这项研究是为了确定成骨不全症患者与正常人相比，尿中糖胺聚糖的排泄模式是否不同。通过三种不同方法比较了定量排泄情况，并通过电泳比较了排泄出的糖胺聚糖的定性组成。通过大分子糖醛酸、尿中成分结合的阿尔辛蓝或通过分配实际分离出的糖胺聚糖量来衡量，正常个体和患病个体排泄的糖胺聚糖量没有差异。然而，患病个体中阿尔辛蓝结合量相对于大分子糖醛酸有统计学上的显著增加，而正常组未出现这种情况，这可能表明成骨不全症患者排泄出了一种能够结合阿尔辛蓝但不是糖胺聚糖的物质。通过电泳检测，两组之间糖胺聚糖排泄在定性上没有差异。
