Omar N M, El-Rabbat N A
J Pharm Sci. 1980 Jan;69(1):24-7. doi: 10.1002/jps.2600690107.
A convenient spectrophotometric determination of diethylstilbestrol and diethylstilbestrol dipropionate was developed involving their interaction with triphenyltetrazolium chloride at 50 degrees for 45 min and subsequent measurement of the formazan formed. The significance of extended conjugation within t,e 4,4'-stilbenediol molecule to induce the color reaction is documented. Ideal adherence of color absorption to Beer's law permitted accurate and precise determination of diethylstilbestrol and diethylstilbestrol dipropionate pure forms over the range of 2-22 microgram of diethylstilbestrol/ml. Application of the tetrazolium color reaction to the analysis of diethylstilbestrol dipropionate dosage forms was achieved without prior hydrolysis or extraction.
开发了一种简便的分光光度法来测定己烯雌酚和己烯雌酚二丙酸酯，该方法包括它们在50℃下与氯化三苯四氮唑反应45分钟，随后测定生成的甲臜。记录了4,4'-二羟基二苯乙烯分子内延长共轭对诱导显色反应的重要性。颜色吸收符合比尔定律，从而能够在2-22微克己烯雌酚/毫升的范围内准确、精确地测定己烯雌酚和己烯雌酚二丙酸酯的纯品形式。无需预先水解或萃取，即可将四氮唑显色反应应用于己烯雌酚二丙酸酯剂型的分析。
