隐匿巴贝斯虫的体外培养

In vitro cultivation of Babesia occultans.

作者信息

Van Niekerk C J, Zweygarth E

机构信息

Protozoology Division, Onderstepoort Veterinary Institute, South Africa.

出版信息

Onderstepoort J Vet Res. 1996 Sep;63(3):259-61.

PMID:8917864

Abstract

Babesia occultans, the causative agent of a benign form of cattle babesiosis in South Africa, was continuously cultivated in microaerophilous stationary-phase culture. A modified medium, 199, supplemented with either 40% (v/v) bovine or 40% (v/v) horse serum, was used. Cultures were initiated in a humidified atmosphere containing 2% O2, 5% CO2, and 93% N2. The highest percentage of parasitized erythrocytes (PPE) reached 4.5% in horse-serum- and 2.4% in bovine-serum-supplemented medium. Parasite suspensions were cryopreserved and successfully resuscitated.

摘要

隐匿巴贝斯虫是南非一种良性牛巴贝斯虫病的病原体，已在微需氧固定相培养中持续培养。使用了改良的199培养基，添加40%（v/v）牛血清或40%（v/v）马血清。培养在含有2%氧气、5%二氧化碳和93%氮气的湿润气氛中开始。在添加马血清的培养基中，被寄生红细胞的最高百分比（PPE）达到4.5%，在添加牛血清的培养基中为2.4%。寄生虫悬液被冷冻保存并成功复苏。