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凝集素研究。三十四。热处理对小扁豆(Lens esuclenta Moench)种子凝集素的影响。

Studies on lectins. XXXIV. The effect of thermal treatment on the lectin of lentil seeds (Lens esuclenta Moench).

作者信息

Körbelová I, Tichá M, Kocourek J

出版信息

Acta Biol Med Ger. 1978;37(3):487-91.

PMID:735619
Abstract

The lectin of lentil seeds (Lens esculenta Moench) dissociates into subunits upon incubation of its solution at temperatures above 50 degrees C. The dissociation is accompanied by precipitation of the heavy subunit, release of Mn2+ from the protein molecule and an almost complete loss of erythroagglutinating activity. Addition of Mn2+ or D-glucose to the lectin solutions has a partially protective effect on the dissociation and inactivation.

摘要

小扁豆种子(兵豆属蒙奇豆)凝集素在温度高于50摄氏度时孵育其溶液会解离成亚基。这种解离伴随着重亚基的沉淀、Mn2+从蛋白质分子中的释放以及红细胞凝集活性几乎完全丧失。向凝集素溶液中添加Mn2+或D-葡萄糖对解离和失活有部分保护作用。

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