Danielova L T, Ambartsumian L A
Antibiotiki. 1980 Jan;25(1):41-4.
Distribution of bacitracin in rabbits when given orally was studied. It was found that bacitracin was absorbed from the gastrointestinal tract, penetrated into the organs and tissues, was partially bound there and rapidly excreted with the urine and bile. The highest percentage of the antibiotic binding was registered in the bone marrow and organs of the lymphoid system. Since the binding of the antibiotic was selective (only in the organs of the lymphoid system) and its presence in the muscle tissue and subproducts (liver, kidneys, etc.) was in the trace amounts, they do not prevent the use of such products in food. Thermal treatment of such products eliminates the antibiotic activity which is also in favour of their use. Therefore, bacitracin may be widely used as a growth stimulator in cattle breeding. For detection of the bacitracin bound fraction in the organs, tissues and animal products it is recommended to use enzymatic hydrolysis of the biological substrates containing bacitracin with a 2 per cent pepsin solution prepared in phosphate buffer, pH 6.0-6.2.
研究了口服杆菌肽在兔体内的分布情况。发现杆菌肽可从胃肠道吸收，渗透到器官和组织中，在那里部分结合，并迅速经尿液和胆汁排出。抗生素结合率最高的是在骨髓和淋巴系统的器官中。由于抗生素的结合具有选择性（仅在淋巴系统的器官中），且其在肌肉组织和副产物（肝脏、肾脏等）中的含量为痕量，因此它们不妨碍此类产品用于食品。对此类产品进行热处理可消除抗生素活性，这也有利于它们的使用。因此，杆菌肽可广泛用作养牛业的生长促进剂。为检测器官、组织和动物产品中结合的杆菌肽部分，建议使用在pH 6.0 - 6.2的磷酸盐缓冲液中配制的2%胃蛋白酶溶液对含杆菌肽的生物底物进行酶水解。
