Danielova L T, Ambartsumian L A
Antibiotiki. 1981 Oct;26(10):769-73.
The possibility of grisin binding in animal organs and tissues was studied and more accurate methods for determination of grisin in animal foods were developed. Such products as meat, milk, fat, eggs and subproducts (liver, kidneys, lungs), as well as organs and tissues of rabbits were tested. The antibiotic was added to the feed in an amount of 400 mg per head. The animals and poultry were killed 1 hour after the feeding. It was shown that grisin was partially bound in vitro and in vivo in the organs and tissues and could not be detected with the routine methods. A procedure for enzymatic hydrolysis of the homogenates with pancreatin, a proteolytic enzyme was elaborated by the authors for determination of the adsorbed or bound antibiotic. The procedure of the enzymatic hydrolysis used alone or in combination with thermal treatment provided determination of 90-100 per cent of grisin contained in the products, while the routine method of homogenization without thermal treatment revealed only 50-60 per cent (or 20-35 per cent in the liver). The use of the procedure increased the accuracy of the grisin traces determination 2.8-15.6 times as compared to the routine methods. It allowed one to obtain more correct data on the true levels of the drug in organs and tissues.
研究了grisin在动物器官和组织中的结合可能性，并开发了更精确的测定动物食品中grisin的方法。对肉类、牛奶、脂肪、鸡蛋及副产品（肝脏、肾脏、肺）以及兔子的器官和组织等产品进行了测试。以每头400毫克的量将抗生素添加到饲料中。喂食1小时后宰杀动物和家禽。结果表明，grisin在体外和体内会部分结合在器官和组织中，常规方法无法检测到。作者精心制定了一种用胰蛋白酶（一种蛋白水解酶）对匀浆进行酶水解的程序，用于测定吸附或结合的抗生素。单独使用或与热处理相结合的酶水解程序可测定产品中90% - 100%的grisin，而未经热处理的常规匀浆方法仅能检测到50% - 60%（肝脏中为20% - 35%）。与常规方法相比，该程序将grisin痕量测定的准确性提高了2.8 - 15.6倍。它使人们能够获得关于器官和组织中药物真实含量的更准确数据。
