Kessel D
Cancer Res. 1980 Feb;40(2):322-4.
Exposure of murine leukemia L1210 cells to graded doses of 5-fluorouracil for 24 hr led to a progressive increase in cell surface hydrophobicity, inhibition of cell division, an an increased cell volume. Among the effects associated with fluorouracil treatment were inhibition of thymidylate synthetase, decreased incorporation of leucine into glycoprotein, and an apparently increased incorporation of thymidine into DNA and of glucosamine into glycoprotein. The latter effects are believed to be caused by depleted metabolite pools. Short-term treatment of L1210 cells with the drug altered only levels of thymidylate synthetase. Cell surface changes therefore appear to be related to long-term effects of fluorouracil associated with impaired synthesis of membrane glycoprotein.
将鼠白血病L1210细胞暴露于不同剂量的5-氟尿嘧啶中24小时，会导致细胞表面疏水性逐渐增加、细胞分裂受到抑制以及细胞体积增大。与氟尿嘧啶处理相关的效应包括胸苷酸合成酶的抑制、亮氨酸掺入糖蛋白的减少，以及胸苷掺入DNA和葡糖胺掺入糖蛋白的明显增加。后一种效应被认为是由代谢物池的耗尽引起的。用该药物对L1210细胞进行短期处理仅改变了胸苷酸合成酶的水平。因此，细胞表面的变化似乎与氟尿嘧啶与膜糖蛋白合成受损相关的长期效应有关。
