Miners J O, Foenander T, Birkett D J
Clin Chem. 1980 Jan;26(1):117-9.
We report a "high-performance" liquid-chromatographic method for measuring 5-fluorocytosine in plasma and cerebrospinal fluid. After deproteinization with trichloroacetic acid, the supernates are chromatographed on a reversed-phase (C18) column. Response to concentration is linear in the range of 5 to 200 mg/L, with ultraviolet detection at 276 nm. The assay requires only 0.1 mL of plasma, is reproducible, and may be performed in less than 12 min. 5-Fluorocytosine concentrations determined by this procedure correlated well with those obtained by spectrofluorometry, although the present method is more specific with no observable interference from co-administered amphotericin B and most other commonly encountered drugs, including salicylat:. This method is applicable to the routine therapeutic monitoring of pediatric and adult patients as well as to pharmacokinetic studies.
我们报告了一种用于测定血浆和脑脊液中5-氟胞嘧啶的“高效”液相色谱法。用三氯乙酸进行脱蛋白处理后，将上清液在反相（C18）柱上进行色谱分析。在5至200mg/L范围内，浓度与响应呈线性关系，于276nm处进行紫外检测。该测定法仅需0.1mL血浆，具有可重复性，且可在不到12分钟内完成。尽管本方法更具特异性，与联合使用的两性霉素B和大多数其他常见药物（包括水杨酸盐）无明显干扰，但通过本方法测定的5-氟胞嘧啶浓度与荧光分光光度法获得的浓度相关性良好。该方法适用于儿科和成人患者的常规治疗监测以及药代动力学研究。
