用于测量铁螯合酶活性的改进放射化学方法。

Improved radiochemical method for measuring ferrochelatase activity.

作者信息

Williams H L, Johnson D J, Haut M J, Slater S, Brennan R G, Altstatt L B

出版信息

Clin Chem. 1980 Jan;26(1):153-6.

Abstract

We describe a radiochemical method for measuring ferrochelatase activity in the in vitro incorporation of iron into mesoporphyrin-IX to form mesoheme. 59Fe is used to quantify ferrochelatase activity in rat liver. The uptake of iron is linearly related to the time allowed for it to occur, and the reaction proceeds optimally under reducing and anerobic conditions, maintained in sealed lyophilization vials under a positive pressure of nitrogen.

摘要

我们描述了一种放射化学方法，用于在体外将铁掺入中卟啉-IX以形成中血红素的过程中测量亚铁螯合酶活性。使用⁵⁹Fe来定量大鼠肝脏中的亚铁螯合酶活性。铁的摄取与允许其发生的时间呈线性相关，并且该反应在还原和厌氧条件下最佳进行，通过在氮气正压下密封的冻干小瓶中维持。