一种用于血红素合酶活性的放射化学测定法。
A radiochemical assay for heme synthase activity.
作者信息
Bloomer J R, Morton K O
出版信息
Enzyme. 1982;28(2-3):220-32. doi: 10.1159/000459104.
A radiochemical assay for heme synthase (ferrochelatase) activity is described in this report. The principle of the assay is to measure the incorporation of 59Fe, added to the reaction flask with a known amount of iron, into heme. Iron is maintained in the ferrous state, and oxygen is excluded from the flask during the incubation of the substrates with enzyme. Labeled heme product is extracted by solvent partitioning and counted in a gamma scintillation spectrometer. The assay can detect picomole amounts of product and is most useful when low levels of heme synthase activity are present.
本报告描述了一种用于血红素合酶（亚铁螯合酶）活性的放射化学测定法。该测定法的原理是测量添加到反应瓶中与已知量铁混合的59Fe掺入血红素中的情况。铁保持在亚铁状态，在底物与酶孵育期间，反应瓶中要排除氧气。标记的血红素产物通过溶剂分配法提取，并在γ闪烁光谱仪中计数。该测定法可检测皮摩尔量的产物，当血红素合酶活性水平较低时最为有用。
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