Liu T Z, Oka K H
Clin Chem. 1980 Jan;26(1):69-71.
We describe a simple, economical procedure for rapidly detecting acetaminophen in serum or plasma. The method is based upon the reduction by the drug of ferric 2,4,6-tris(2-pyridyl)-s-triazine, at an acidic pH, to ferrous 2,4,6-tris(2-pyridyl)-S-triazine complex, which absorbs maximally at 593 nm. Absorbance and acetaminophen concentration are linearly related from 25 to 400 mg/L, and so therapeutic and toxic concentrations can be measured. The method is accurate; day-to-day CV's for two pooled control specimens (103 and 227 mg/L) were 4.4 and 6.6%. Correlation studies, with an established nitration method and with the free-radical diphenylpicrylhydrazyl dye method, showed correlation coefficients of 0.985 and 0.915, respectively. Of 25 commonly used drugs tested, only levodopa, oxyphenylbutazone, and phenylephrine interfere significantly. Interference from salicylate, salicylamide, and phenylbutazone was insignificant.
我们描述了一种用于快速检测血清或血浆中对乙酰氨基酚的简单、经济的方法。该方法基于在酸性pH条件下，药物将三（2 - 吡啶基）-s-三嗪铁（III）还原为三（2 - 吡啶基）-s-三嗪亚铁（II）络合物，该络合物在593nm处有最大吸收。吸光度与对乙酰氨基酚浓度在25至400mg/L范围内呈线性相关，因此可以测量治疗浓度和中毒浓度。该方法准确；两个混合对照样本（103和227mg/L）的日间变异系数分别为4.4%和6.6%。与既定的硝化法和自由基二苯基苦味酰基肼染料法的相关性研究表明，相关系数分别为0.985和0.915。在测试的25种常用药物中，只有左旋多巴、羟基保泰松和去氧肾上腺素有显著干扰。水杨酸盐、水杨酰胺和保泰松的干扰不显著。
