Hale P W, Poklis A
J Anal Toxicol. 1983 Sep-Oct;7(5):249-51. doi: 10.1093/jat/7.5.249.
An evaluation of a new colorimetric assay (SIGMA-430) for the rapid determination of acetaminophen in serum or plasma was performed. Acetaminophen was reacted with nitrous acid to form a 2-nitro-5-acetamidophenol derivative, which was measured colorimetrically in an alkaline medium. The assay was linear over a concentration range of 50-500 mg/L. Within-run and between-run CV's with a 300 mg/L sample were 1.51% (n = 12) and 3.00% (n = 20), respectively. Mean analytical recovery of acetaminophen added to plasma at 50-600 mg/L was 102.0%. Seventeen commonly prescribed weakly acidic or neutral drugs were also analyzed by the method with no significant interferences. A comparison of 21 consecutive serum samples with the colorimetric assay and an HPLC method yielded a correlation coefficient of 0.989. Colorimetric values were statistically higher.
对一种用于快速测定血清或血浆中对乙酰氨基酚的新型比色测定法（SIGMA - 430）进行了评估。对乙酰氨基酚与亚硝酸反应形成2 - 硝基 - 5 - 乙酰氨基苯酚衍生物，该衍生物在碱性介质中进行比色测定。该测定法在50 - 500 mg/L的浓度范围内呈线性。300 mg/L样品的批内和批间变异系数分别为1.51%（n = 12）和3.00%（n = 20）。添加到血浆中浓度为50 - 600 mg/L的对乙酰氨基酚的平均分析回收率为102.0%。该方法还对17种常用的弱酸性或中性药物进行了分析，无明显干扰。对21份连续血清样品采用比色测定法和高效液相色谱法进行比较，相关系数为0.989。比色测定值在统计学上更高。
