Melera P W, Wolgemuth D, Biedler J L, Hession C
J Biol Chem. 1980 Jan 25;255(2):319-22.
A comparison by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the proteins synthesized by antifolate-sensitive (DC-3F) and -resistant (DC-3F/A3 and DC-3F/MQ19) Chinese hamster lung fibroblast cells has shown that an apparent molecular weight difference exists between the dihydrofolate reductases overproduced by the resistant sublines. Translation in a rabbit reticulocyte lysate system of polysomal and cytoplasmic mRNAs from the three sublines has shown that the overproduction of dihydrofolate reductase by resistant cells is accompanied by increased levels of dihydrofolate reductase-specific mRNA and that the different molecular weight forms of dihydrofolate reductase are encoded by their respective mRNAs.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对叶酸拮抗剂敏感（DC-3F）和耐药（DC-3F/A3及DC-3F/MQ19）的中国仓鼠肺成纤维细胞合成的蛋白质进行比较，结果表明，耐药亚系过量产生的二氢叶酸还原酶之间存在明显的分子量差异。对这三个亚系的多聚核糖体和细胞质mRNA在兔网织红细胞裂解物系统中进行翻译，结果显示，耐药细胞中二氢叶酸还原酶的过量产生伴随着二氢叶酸还原酶特异性mRNA水平的升高，且不同分子量形式的二氢叶酸还原酶由各自的mRNA编码。
