γ射线照射后二氢叶酸还原酶基因座遗传改变的直接证明。
Direct demonstration of genetic alterations at the dihydrofolate reductase locus after gamma irradiation.
作者信息
Graf L H, Chasin L A
出版信息
Mol Cell Biol. 1982 Jan;2(1):93-6. doi: 10.1128/mcb.2.1.93-96.1982.
Abstract
Gamma ray-induced mutants of Chinese hamster ovary cells lacking dihydrofolate reductase activity were screened for DNA sequence changes at the locus specifying this activity by using a cloned cDNA probe. Two of nine mutants screened displayed an altered restriction fragment pattern suggesting the occurrence of DNA deletions or rearrangements.
摘要
利用克隆的互补DNA（cDNA）探针，对缺乏二氢叶酸还原酶活性的中国仓鼠卵巢细胞的伽马射线诱导突变体进行筛选，以确定在指定该活性的基因座处的DNA序列变化。在筛选的9个突变体中，有2个显示出限制性片段模式改变，提示发生了DNA缺失或重排。
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