Hammarström S
J Biol Chem. 1980 Jan 25;255(2):518-21.
Partially purified thromboxane synthase from human platelets converted prostaglandin G2 to two products. These were transformed to 12-hydroxy-5,8,10-heptadecatrienoic acid and thromboxane B2 by stannous chloride reduction and to 12-keto-5,8,10-heptadecatrienoic acid and 15-ketothromboxane B2 by lead tetraacetate dehydration. Trapping with methanol after short incubations of substrate with enzyme and reduction by stannous chloride yielded 11-O-methylthromboxane B2. Based on these results, the enzymatically formed products were identified as 12-hydroperoxy-5,8,10-heptadecatrienoic acid and 15-hydroperoxythromboxane A2.
从人血小板中部分纯化得到的血栓素合酶可将前列腺素G2转化为两种产物。通过氯化亚锡还原，这些产物转化为12-羟基-5,8,10-十七碳三烯酸和血栓素B2；通过四乙酸铅脱水，它们转化为12-酮基-5,8,10-十七碳三烯酸和15-酮基血栓素B2。在底物与酶短暂孵育后用甲醇捕获，并经氯化亚锡还原，得到11-O-甲基血栓素B2。基于这些结果，酶促形成的产物被鉴定为12-氢过氧-5,8,10-十七碳三烯酸和15-氢过氧血栓素A2。
