Korn J H, Halushka P V, LeRoy E C
J Clin Invest. 1980 Feb;65(2):543-54. doi: 10.1172/JCI109698.
The role of immune cell products in modulating connective tissue metabolism was investigated. Supernates of both unstimulated and phytohemagglutinin-stimulated human mononuclear cell cultures suppressed fibroblast proliferation (up to 90%) and concomintantly stimulated fibroblast prostaglandin E(PGE) synthesis (20- to 70-fold). The growth suppression was, at least in part, a secondary result of the increased fibroblast PGE synthesis; growth suppression (a) paralled the increased fibroblast PGE synthesis, (b) was reversed by addition of inhibitors of prostaglandin synthesis (indomethacin, meclofenamate, and eicostaetraynoic acid), and (c) was reproduced by addition of exogenous PGE2 to fibroblast cultures. The prostaglandin-stimulatory, growth-suppressive activity was a product of non-T-lymphocyte, adherent cells and was present within 6 h of mononuclear cell culture. The activity was heat (56 degrees C) and trypsin sensitive, nondialyzable, and appeared in the 12,000-20,000 mol wt fractions by Sephadex G-100 chromatography. The activity in supernates of mononuclear cell cultures was removed by incubation with fibroblasts but not by similar incubation with peripheral blood mononuclear cells. Mononuclear cells release a factor(s) which modulates fibroblast proliferation by altering prostaglandin metabolism.
研究了免疫细胞产物在调节结缔组织代谢中的作用。未刺激的和经植物血凝素刺激的人单核细胞培养物的上清液均抑制成纤维细胞增殖（高达90%），并同时刺激成纤维细胞前列腺素E（PGE）的合成（增加20至70倍）。生长抑制至少部分是成纤维细胞PGE合成增加的继发结果；生长抑制（a）与成纤维细胞PGE合成增加平行，（b）通过添加前列腺素合成抑制剂（吲哚美辛、甲氯芬那酸和二十碳四烯酸）而逆转，（c）通过向成纤维细胞培养物中添加外源性PGE2而重现。前列腺素刺激、生长抑制活性是非T淋巴细胞贴壁细胞的产物，在单核细胞培养6小时内即可出现。该活性对热（56℃）和胰蛋白酶敏感，不可透析，通过Sephadex G - 100层析出现在分子量为12,000 - 20,000的组分中。单核细胞培养物上清液中的活性通过与成纤维细胞孵育而去除，但与外周血单核细胞进行类似孵育则不能去除。单核细胞释放一种因子，该因子通过改变前列腺素代谢来调节成纤维细胞增殖。
