Leibovich S J, Ross R
Am J Pathol. 1976 Sep;84(3):501-14.
Whole blood serum (HBS) stimulates the proliferation of fibroblasts in vitro, while platelet-poor plasma serum (PPPS) does not. Fibroblasts grown in the presence of PPPS are truly quiescent in that they are not deprived of nutrients in the culture medium and less than 3% of the cells synthesize DNA and divide. In vivo experiments have suggested that macrophages are necessary for stimulation of fibroplasia during wound repair. We have utilized the difference in growth-promoting activity between HBS and PPPS to study the ability of macrophages to produce growth-promoting activity in cell culture. Guinea pig peritoneal macrophages cultured in vitro in medium containing PPPS release into the medium, either directly or indirectly, a factor (or factors) that stimulates the proliferation of guinea pig wound fibroblasts. This macrophage-dependent, fibroblast-stimulating activity (MFSA) is nondialyzable, heat stable (56 C for 30 minutes), and requires culture in vitro for demonstration of activity. The relationship between MFSA and other growth factor(s) has not yet been determined. In contrast to the macrophage, lymphocytes prepared from mesenteric lymph nodes produced no figroblast-stimulating activity.
全血血清（HBS）可在体外刺激成纤维细胞增殖，而少血小板血浆血清（PPPS）则不能。在PPPS存在的情况下生长的成纤维细胞确实处于静止状态，因为它们在培养基中没有缺乏营养，并且少于3%的细胞合成DNA并分裂。体内实验表明，巨噬细胞是伤口修复过程中刺激纤维增生所必需的。我们利用HBS和PPPS之间促生长活性的差异来研究巨噬细胞在细胞培养中产生促生长活性的能力。在含有PPPS的培养基中体外培养的豚鼠腹腔巨噬细胞直接或间接地向培养基中释放一种（或多种）刺激豚鼠伤口成纤维细胞增殖的因子。这种依赖巨噬细胞的成纤维细胞刺激活性（MFSA）不可透析、热稳定（56℃ 30分钟），并且需要体外培养以证明其活性。MFSA与其他生长因子之间的关系尚未确定。与巨噬细胞相反，从肠系膜淋巴结制备的淋巴细胞没有产生成纤维细胞刺激活性。
