Berman B, Gigli I
J Immunol. 1980 Feb;124(2):685-90.
A population of guinea pig epidermal cells possessed the ability to form rosettes with IgM antibody-sensitized sheep erythrocytes coated with purified human C3b (SEAIgMC1-), but not with intermediates carrying C1, C4, or C2. This population of rosette-forming epidermal cells was identified as Langerhans cells (LC) ultrastructurally and by LC depletion and enrichment studies. In addition, these cells were able to bind fluid phase C3b, which inhibited any subsequent SEAIgMC1-3b rosette formation. Rosette formation was also inhibited after SEAIgMC1-3b pretreatment with beta 1H and C3b-inactivator. LC were shown to possess trypsin-resistant C3b and Fc receptors, and unlike the temperature-independent nature of LC Fc-rosette formation, the ability of LC to form C3 rosettes was a temperature-dependent process. The finding of C3b receptors on the surface of guinea pig epidermal LC supports the concept that they are of the phagocytic monocyte-macrophage family.
豚鼠表皮细胞群体具有与包被纯化人C3b的IgM抗体致敏绵羊红细胞（SEAIgMC1-）形成玫瑰花结的能力，但与携带C1、C4或C2的中间产物则不能形成。通过超微结构以及LC耗竭和富集研究，这群形成玫瑰花结的表皮细胞被鉴定为朗格汉斯细胞（LC）。此外，这些细胞能够结合液相C3b，这会抑制随后任何SEAIgMC1-3b玫瑰花结的形成。在用β1H和C3b灭活剂预处理SEAIgMC1-3b后，玫瑰花结形成也受到抑制。LC被证明具有抗胰蛋白酶的C3b和Fc受体，与LC Fc玫瑰花结形成的温度无关特性不同，LC形成C3玫瑰花结的能力是一个温度依赖性过程。豚鼠表皮LC表面存在C3b受体这一发现支持了它们属于吞噬性单核细胞 - 巨噬细胞家族的概念。
