Kemp W M, Brown P R, Merritt S C, Miller R E
J Immunol. 1980 Feb;124(2):806-11.
Adult Schistosoma mansoni from mice were incubated in rabbit anti-mouse immunoglobulin class or subclass-specific sera. The rabbit antibody binding was then visualized by incubation of the labeled worms in fluoresceinated Staphylococcus aureus, which associated with the Fc portion of the rabbit antibody molecule by means of bacterial cell wall Protein A. The assay system was found to be simple, inexpensive, specific, repeatable, and rapidly accomplished. By using this technique, previous work documenting the presence of mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM on the tegumental surface of adult worms of mouse origin was confirmed. It was further confirmed that the complexing of rabbit antibody to the mouse antigen led to the shedding of that antigen from the parasite's surface membrane within 20 min at 37 degrees C. This shedding phenomenon was specific for only those antigens interacting with the ligand and was inhibited by incubation with sodium fluoride, 2-deoxy-D-glucose, cytochalasin B, cytochalasin D, caffeine, or at 4 degrees C. These data indicate a sophisticated control mechanism exercised by the parasite over its interface with the host and may indicate a complexity of the host-parasite relationship not heretofore recognized.
将来自小鼠的成年曼氏血吸虫置于兔抗小鼠免疫球蛋白类或亚类特异性血清中进行孵育。然后，通过将标记的虫体与荧光素标记的金黄色葡萄球菌一起孵育来观察兔抗体的结合情况，金黄色葡萄球菌借助细菌细胞壁蛋白A与兔抗体分子的Fc部分相结合。结果发现该检测系统简单、廉价、特异、可重复且完成迅速。通过使用该技术，先前关于小鼠来源的成年虫体表膜上存在小鼠IgG1、IgG2a、IgG2b、IgG3、IgA和IgM的研究得到了证实。进一步证实，兔抗体与小鼠抗原的结合在37℃下20分钟内导致该抗原从寄生虫的表面膜脱落。这种脱落现象仅针对那些与配体相互作用的抗原具有特异性，并可通过与氟化钠、2-脱氧-D-葡萄糖、细胞松弛素B、细胞松弛素D、咖啡因一起孵育或在4℃下孵育而受到抑制。这些数据表明寄生虫对其与宿主的界面行使着复杂的控制机制，并且可能表明宿主-寄生虫关系存在此前未被认识到的复杂性。
