Biochemistry of metalocenes. The organ distribution of hydroxyacetyl [103Ru]ruthenocene and its glucuronide in mice.

Hydroxyacetyl [103Ru]ruthenocene and its o-glucuronide were prepared in vitro by incubation of acetyl [103Ru]ruthenocene with rat-liver homogenate, NADPH, and UDP-glucuronate. The factors affecting hydroxylation and glucuronidation in vitro were optimized for acetylruthenocene. Hydroxyacetyl [103Ru]ruthenocene glucuronide showed no affinity for the adrenal glands, but after i.v. administration of hy droxyacetyl [103Ru]ruthenocene there was a distinct accumulation of Ru-103 in adrenals, similar to that found after administration of acetyl [103Ru]ruthenocene.