人血浆中6-硫鸟嘌呤的测定
Assay of 6-thioguanine in human plasma.
作者信息
Dooley T, Maddocks J L
出版信息
Br J Clin Pharmacol. 1980 Jan;9(1):77-82. doi: 10.1111/j.1365-2125.1980.tb04800.x.
Abstract
- A fluorimetric method has been developed for measuring therapeutic concentrations of 6-thioguanine in human plasma. The drug was measured as an oxidized derivative, guanine 6-sulphonate. 2. 6-thioguanine was extracted from plasma by a novel procedure which isolated the drug from an interfering plasma components. This method involved the formation of the thioguanine phenyl mercury derivative in alkaline plasma and its extraction into toluene. The free drug was released by back-extraction into hydrochloric acid. 3. The assay is specific and shows a limit of sensitivity of 5 ng/ml. This was shown to be adequate for measuring plasma concentrations of 6-thioguanine after the administration of a 160 mg dose in a patient with leukaemia.
摘要
- 已开发出一种荧光法用于测定人血浆中6-硫鸟嘌呤的治疗浓度。该药物作为氧化衍生物鸟嘌呤6-磺酸盐进行测定。2. 通过一种新方法从血浆中提取6-硫鸟嘌呤，该方法可将药物与干扰性血浆成分分离。此方法包括在碱性血浆中形成硫鸟嘌呤苯基汞衍生物并将其萃取到甲苯中。通过反萃取到盐酸中释放出游离药物。3. 该测定法具有特异性，灵敏度极限为5 ng/ml。在一名白血病患者给予160 mg剂量后，已证明该灵敏度足以测定血浆中6-硫鸟嘌呤的浓度。
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