Lihou M G, Smith P J
Br J Cancer. 1983 Oct;48(4):559-67. doi: 10.1038/bjc.1983.229.
A system for the prediction of clinical response in acute myelocytic leukaemia (AML), based on inhibition of growth of colony forming cells (CFC) was studied. If the product of initial drug concentration and time of exposure (C X T) was constant, the response to adriamycin (Adr) was constant, at T values less than 48 h. No constancy of response to the phase-specific agents cytosine arabinoside (Ara-C) and 6-thioguanine (6TG) was demonstrated with constant C X T (T value range 0.25-48 h). Hence in the predictive test, a 1 h incubation with Adr was employed whilst a continuous exposure to Ara-C and 6TG, with these drugs incorporated in the agar medium, was used. The in vitro sensitivity to Adr, Ara-C and 6TG of 19 AML patients and the predictive value of several parameters of sensitivity were evaluated. 6TG sensitivity was not useful for prediction of remission. Adr sensitivity in vitro made a greater contribution to prediction of remission than did Ara-C sensitivity. Seventy-nine percent of patients were correctly classified if Adr data alone were considered. A multivariate function including Adr and Ara-C results was obtained which resulted in 84% of patients correctly classified as sensitive or resistant to the agents received in remission-induction therapy.
研究了一种基于抑制集落形成细胞（CFC）生长来预测急性髓细胞白血病（AML）临床反应的系统。当初始药物浓度与暴露时间的乘积（C×T）恒定时，在T值小于48小时的情况下，对阿霉素（Adr）的反应是恒定的。对于细胞周期特异性药物阿糖胞苷（Ara-C）和6-硫鸟嘌呤（6TG），在C×T恒定（T值范围为0.25 - 48小时）时，未显示出反应的恒定性。因此，在预测试验中，采用与Adr孵育1小时，而使用将Ara-C和6TG掺入琼脂培养基中的持续暴露方式。评估了19例AML患者对Adr、Ara-C和6TG的体外敏感性以及几个敏感性参数的预测价值。6TG敏感性对缓解预测无用。体外Adr敏感性对缓解预测的贡献比Ara-C敏感性更大。如果仅考虑Adr数据，79%的患者能被正确分类。获得了一个包含Adr和Ara-C结果的多变量函数，该函数使84%的患者被正确分类为对缓解诱导治疗中所接受药物敏感或耐药。
