Gillett M P, Costa E M, Owen J S
Biochim Biophys Acta. 1980 Feb 22;617(2):237-44. doi: 10.1016/0005-2760(80)90166-6.
Mouse, rat and human plasma were exposed to minimum concentrations of disulphide or minimum pre-incubation at 55 degrees C in order to inhibit lecithin : cholesterol acyltransferase activity completely. The plasma samples were subsequently incubated at 37 degrees C and changes in individual phospholipid concentrations determined. Significant utilization of phosphatidylcholine and formation of lysophosphatidylcholine occurred only in disulphide-treated mouse plasma and this was accompanied by a decrease in total phospholipid concentration. When disulphide-treated mouse plasma was incubated with [U-14C]phosphatidylcholine radioactivity was additionally recovered in the lysophosphatidylcholine, non-esterified fatty acid and glycero-3-phosphocholine fractions; maximum conversion occurred at close to physiological pH. These observations suggest that phospholipase A and lysophosphatidylcholine hydrolase enzymes are active in mouse plasma but that phospholipase A is either absent or inactive in rat and human plasma.
将小鼠、大鼠和人的血浆暴露于最低浓度的二硫化物中，或在55摄氏度下进行最短时间的预孵育，以完全抑制卵磷脂胆固醇酰基转移酶的活性。随后将血浆样品在37摄氏度下孵育，并测定各个磷脂浓度的变化。只有在经二硫化物处理的小鼠血浆中才发生了显著的磷脂酰胆碱利用和溶血磷脂酰胆碱的形成，并且这伴随着总磷脂浓度的降低。当将经二硫化物处理的小鼠血浆与[U-14C]磷脂酰胆碱一起孵育时，在溶血磷脂酰胆碱、非酯化脂肪酸和甘油-3-磷酸胆碱组分中还回收了放射性；在接近生理pH值时发生最大转化。这些观察结果表明，磷脂酶A和溶血磷脂酰胆碱水解酶在小鼠血浆中具有活性，但磷脂酶A在大鼠和人的血浆中要么不存在，要么无活性。
