Jung K, Köhler A
Clin Chim Acta. 1980 Feb 14;101(1):1-4. doi: 10.1016/0009-8981(80)90049-2.
In activity determination of alkaline phosphatase (AP), measuring temperature, type and concentration of buffer, and protein concentration in the test influence the molar absorptivity of 4-nitrophenol. Thus systematic errors of up to 3% may occur in activity determinations of AP if these influences are not taken into account.
在碱性磷酸酶（AP）活性测定中，测量温度、缓冲液的类型和浓度以及测试中的蛋白质浓度会影响4-硝基苯酚的摩尔吸光系数。因此，如果不考虑这些影响因素，AP活性测定中可能会出现高达3%的系统误差。
