Mraz W, Schwarzmann G, Sattler J, Momoi T, Seemann B, Wiegandt H
Hoppe Seylers Z Physiol Chem. 1980;361(2):177-85. doi: 10.1515/bchm2.1980.361.1.177.
The gangliosides GLac1, GGtri1 and GGtet1 in aqueous medium sediment in the ultracentrifuge as oligomeric aggregates independent of concentration at 10(-3)M to 10(-9)M with the sedimentation constants S20,W of 5.3, 7.5 and 10.0, respectively. If gangliosides dissolved in dimethylsulfoxide were diluted with water, this resulted in aggregates with lower sedimentation coefficients. In the presence of 1M tetramethylurea no sedimentation of gangliosides could be observed in the ultracentrifuge. A ganglioside analogue (gangliosidoide) with two hydrocarbon chains instead of the ceramide moiety shows a similar sedimentation behaviour as the native compound GGtet1. However, a ganglioside analogue with only one aliphatic hydrocarbon chain forms sedimenting aggregates only at concentrations exceeding 10(-3)M.
神经节苷脂GLac1、GGtri1和GGtet1在水性介质中，于超速离心机中以寡聚聚集体形式沉降，在10⁻³M至10⁻⁹M浓度范围内与浓度无关，其沉降常数S₂₀,W分别为5.3、7.5和10.0。如果将溶解在二甲基亚砜中的神经节苷脂用水稀释，会形成沉降系数较低的聚集体。在1M四甲基脲存在下，在超速离心机中未观察到神经节苷脂的沉降。一种具有两条烃链而非神经酰胺部分的神经节苷脂类似物（神经节苷脂类似物）表现出与天然化合物GGtet1相似的沉降行为。然而，一种仅具有一条脂肪烃链的神经节苷脂类似物仅在浓度超过10⁻³M时形成沉降聚集体。
