Gipson I K, Anderson R A
Invest Ophthalmol Vis Sci. 1980 Apr;19(4):341-9.
Rat corneas with 3 mm central corneal abrasions were organ-cultured in the presence of four plant lectins, concanavalin A (ConA), wheat germ agglutinin (WGA), lotus lectin, and soybean agglutinin (SBA), ConA at 20 and 50 micrograms/ml of culture medium slowed epithelial migration. WGA at 20 and 50 micrograms/ml of medium completely inhibited migration. The effects of the ConA at both 20 and 50 micrograms concentrations and of WGA at 20 micrograms were reversible. Lotus lectin and SBA did not effect motility at concentrations up to and including 50 micrograms/ml of medium. The specific sugar to which SBA binds, N-acetyl-galactosamine, did however, slow epithelial migrations. This study demonstrates that blocking glucose, mannose, and glucosamine sugar moities of glycoproteins on the cell surface and/or basement membrane inhibits epithelial cell migration during healing of corneal abrasions.
对中央角膜有3毫米擦伤的大鼠角膜进行器官培养，培养环境中含有四种植物凝集素：伴刀豆球蛋白A（ConA）、麦胚凝集素（WGA）、莲凝集素和大豆凝集素（SBA）。培养基中ConA浓度为20和50微克/毫升时会减缓上皮细胞迁移。培养基中WGA浓度为20和50微克/毫升时会完全抑制迁移。ConA浓度为20和50微克时以及WGA浓度为20微克时的作用是可逆的。莲凝集素和SBA在培养基浓度高达并包括50微克/毫升时对运动性没有影响。然而，SBA所结合的特定糖类N - 乙酰半乳糖胺确实会减缓上皮细胞迁移。这项研究表明，阻断细胞表面和/或基底膜上糖蛋白的葡萄糖、甘露糖和氨基葡萄糖糖基会抑制角膜擦伤愈合过程中的上皮细胞迁移。
