游离核糖体合成大鼠肝脏微粒体细胞色素b5 。
Synthesis of rat liver microsomal cytochrome b5 by free ribosomes.
作者信息
Rachubinski R A, Verma D P, Bergeron J J
出版信息
J Cell Biol. 1980 Mar;84(3):705-16. doi: 10.1083/jcb.84.3.705.
Abstract
Free and membrane-bound polyribosomes were separated from liver homogenates and characterized by electron microscopy. Using the wheat germ cell-free translation system, total translation products of poly A+RNA extracted from free polyribosomes (poly A+RNAf) showed some correlation to total liver cytosol proteins. In contrast, translation products of poly A+RNA from membrane-bound polyribosomes (poly A+RNAmb) showed some similarity to rat serum. Antibody to purified rat serum albumin immunoprecipitated from only the translation products of poly A+RNAmb a single polypeptide of mol wt 68,000. i.e., 3,000 greater than secreted serum albumin. In contrast, antibody to detergent-extracted cytochrome b5 immunoprecipitated from only the translation products of poly A+RNAf a single polypeptide of mol wt 17,500, identical to that of microsomal cytochrome b5. A consideration of the known properties of cytochrome b5 is consistent with an exclusive site of synthesis on free ribosomes.
摘要
从肝脏匀浆中分离出游离和膜结合多核糖体，并通过电子显微镜进行表征。使用小麦胚芽无细胞翻译系统，从游离多核糖体（poly A+RNAf）中提取的多聚腺苷酸加尾RNA（poly A+RNA）的总翻译产物与肝脏总胞质溶胶蛋白存在一定相关性。相比之下，膜结合多核糖体（poly A+RNAmb）的多聚腺苷酸加尾RNA的翻译产物与大鼠血清有一些相似之处。针对纯化的大鼠血清白蛋白的抗体仅从poly A+RNAmb的翻译产物中免疫沉淀出一种分子量为68,000的单一多肽，即比分泌的血清白蛋白大3,000。相比之下，针对去污剂提取的细胞色素b5的抗体仅从poly A+RNAf的翻译产物中免疫沉淀出一种分子量为17,500的单一多肽，与微粒体细胞色素b5相同。对细胞色素b5已知特性的考虑与在游离核糖体上的专属合成位点一致。
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