Dorfman N A, Civin C I, Wunderlich J R
J Immunol Methods. 1980;32(2):127-39. doi: 10.1016/0022-1759(80)90066-6.
Preparation of target cells from tissue culture lines which grow adherent to tissue culture vessels is often desirable for tests of cell-mediated cytotoxicity (CMC). In the present study we used cells derived from adherent tissue culture lines to compare the merits of suspension vs. adherent target cells in short-term 51Cr-release assays. Cytotoxic activity of murine spleen cells sensitized in vitro against allogeneic spleen cells or syngeneic sarcoma cells was tested with fibroblast or sarcoma target cells. In parallel tests, aliquots of tissue culture lines were detached and used as either suspension or adherent target cells in CMC assays, matching the concentrations of suspension and adherent target cells. In both allogeneic and syngeneic combinations adherent target cells released less 51Cr spontaneously and were more susceptible to CMC than their suspension counterparts.
从贴附于组织培养容器生长的组织培养细胞系中制备靶细胞，常用于细胞介导的细胞毒性（CMC）检测。在本研究中，我们使用来自贴附组织培养细胞系的细胞，在短期51Cr释放试验中比较悬浮靶细胞与贴附靶细胞的优缺点。用成纤维细胞或肉瘤靶细胞检测体外致敏的小鼠脾细胞对同种异体脾细胞或同基因肉瘤细胞的细胞毒活性。在平行试验中，将组织培养细胞系的等分试样分离，并在CMC检测中用作悬浮或贴附靶细胞，使悬浮靶细胞和贴附靶细胞的浓度相匹配。在同种异体和同基因组合中，贴附靶细胞自发释放的51Cr较少，并且比其悬浮对应物更易受到CMC的影响。
