一种用于测量细胞介导的同种异体移植和肿瘤对少量贴壁靶细胞免疫的同位素释放测定法和末端标记测定法。

An isotope-release assay and terminal-labeling assay for measuring cell-mediated allograft and tumor immunity to small numbers of adherent target cells.

作者信息

Tamerius J D, Garrigues H J, Hellström I, Hellström K E

出版信息

J Immunol Methods. 1978;22(1-2):1-22. doi: 10.1016/0022-1759(78)90054-6.

Abstract

A 51Cr-release assay and terminal 51Cr-labeling assay for measuring cell-mediated immunity to adherent target cells is described. Both techniques utilize small 10 microliter-per well microtiter plates, require low numbers of target cells (50-500 per well), and consequently, relatively small numbers of effector cells per well (3x10(3) -1x10(5)). Both assays are objective, quantitative, and simple to perform. The suitability of these techniques for monitoring immunologically specific, cellmediated, cytotoxic response to syngeneic and allogeneic tumor cells and normal skin fibroblasts is demonstrated. Lymph node cells, spleen cells and peritoneal exudate cells serve as effectors.

摘要

本文描述了一种用于测量针对贴壁靶细胞的细胞介导免疫的51Cr释放试验和终末51Cr标记试验。这两种技术均使用每孔10微升的小型微量滴定板，所需靶细胞数量少（每孔50 - 500个），因此每孔效应细胞数量也相对较少（3×10³ - 1×10⁵个）。两种试验均客观、定量且操作简单。证明了这些技术适用于监测对同基因和异基因肿瘤细胞以及正常皮肤成纤维细胞的免疫特异性、细胞介导的细胞毒性反应。淋巴结细胞、脾细胞和腹腔渗出细胞可作为效应细胞。