纤连蛋白由人类巨噬细胞产生。
Fibronectin is produced by human macrophages.
作者信息
Alitalo K, Hovi T, Vaheri A
出版信息
J Exp Med. 1980 Mar 1;151(3):602-13. doi: 10.1084/jem.151.3.602.
Abstract
Monocyte-enriched cultures were prepared from human blood mononuclear leukocytes by adherence to growth substratum. Synthesis and secretion of fibronectin was detected in these cultures concomitantly with morphological differentiation, starting on day 3--5. Production of fibronectin by macrophages was documented by metabolic labeling followed by immunoprecipitation and gel electrophoresis, radioimmunoassay specific for human fibronectin, and by indirect immunofluorescence. Fibronectin was detected mainly intracellularly but was also detected pericellularly only in minute amounts. No production of collagenous proteins was seen in these cultures. Macrophage fibronectin might act in vivo as a nonspecific opsonin and promote cell adhesion during macrophage migration in tissues.
摘要
通过贴壁于生长基质，从人血单核白细胞制备富含单核细胞的培养物。在这些培养物中，从第3 - 5天开始，伴随着形态分化，检测到纤连蛋白的合成与分泌。通过代谢标记，随后进行免疫沉淀和凝胶电泳、针对人纤连蛋白的放射免疫测定以及间接免疫荧光，记录了巨噬细胞产生纤连蛋白的情况。纤连蛋白主要在细胞内被检测到，但仅在极少量的细胞周围也能检测到。在这些培养物中未观察到胶原蛋白的产生。巨噬细胞纤连蛋白在体内可能作为一种非特异性调理素起作用，并在巨噬细胞在组织中迁移期间促进细胞黏附。
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Cross-linking of a major fibroblast surface-associated glycoprotein (fibronectin) catalyzed by blood coagulation factor XIII.由凝血因子 XIII 催化的一种主要的成纤维细胞表面相关糖蛋白（纤连蛋白）的交联。
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