Flewellen E H, Nelson T E
Anesthesiology. 1980 Apr;52(4):303-8. doi: 10.1097/00000542-198004000-00003.
The authors examined the thesis that a dose of dantrolene producing 95 per cent of maximal muscle relaxation (ED95) would effectively prevent or treat malignant hyperthermia (MH). In one group of four pigs sensitive to malignant hyperthermia (MHS) a dose response to intravenous dantrolene was determined by quantitation of toe twitch tension. From these data, the ED95 relaxant dose (3.5 mg/kg) was derived. In a second group of four MHS pigs, the ED95 dantrolene dose was administered prior to MH challenge with succinylcholine, 2 mg/kg, and halothane, 1.5 per cent. MH was prevented in each animal, while measurements of arterial pressure, arterial blood-gas, pH and lactate values, rectal temperature, and heart rate were done. Later, MH rapidly developed in the same four animals when they were again challenged. When treated with the ED95 dose, each animal survived. Finally, each pig was challenged without dantrolene prophylaxis or therapeusis, and all succumbed from MH. Previous studies have shown the efficacious use of dantrolene in prevention or treatment of porcine MH, but doses used have varied, without rationale. The present study shows that in MHS pigs the ED95 muscle relaxant dose of dantrolene (3.5 mg/kg) successfully prevents and treats MH.
作者检验了这样一种论点，即产生最大肌肉松弛度95%的丹曲林剂量（ED95）能有效预防或治疗恶性高热（MH）。在一组四只对恶性高热敏感（MHS）的猪中，通过对趾部抽搐张力进行定量测定，确定静脉注射丹曲林的剂量反应。根据这些数据得出ED95松弛剂量（3.5毫克/千克）。在第二组四只MHS猪中，在使用2毫克/千克琥珀酰胆碱和1.5%氟烷进行MH激发之前，给予ED95剂量的丹曲林。每只动物的MH均得到预防，同时对动脉压、动脉血气、pH值和乳酸值、直肠温度及心率进行了测量。之后，当这四只动物再次受到激发时，MH迅速发作。使用ED95剂量进行治疗时，每只动物均存活。最后，对每头猪在未进行丹曲林预防或治疗的情况下进行激发，所有猪均死于MH。先前的研究已表明丹曲林在预防或治疗猪MH方面有效，但所使用的剂量各不相同，且缺乏理论依据。本研究表明，在MHS猪中，ED95肌肉松弛剂量的丹曲林（3.5毫克/千克）能成功预防和治疗MH。
