Shapiro S H, Wessely Z, Klavins J V
Ann Clin Lab Sci. 1980 Jan-Feb;10(1):45-33.
Ultrastructural changes in mitochondria from hepatocytes of male Swiss Webster mice were observed during the course of porphyria induced by ingestion of a powdered diet containing 2.5 percent griseofulvin (GF). A variety of sporadic mitochondrial alterations were noted between three days and 22 weeks. Mitochondria appeared intact in hepatocytes from control mice fed powdered diet alone. The abundance and prominence of altered mitochondria increased after seven weeks and was particularly apparent after 15 weeks, concurrent with pronounced hepatomegaly and Mallory body (MB) formation. One or more changes observed in some mitochondria of several hepatocytes included: swelling, associated myelin bodies, increased matrix density, disoriented cristae, intracristal swelling, bizarre shapes, paracrystalline inclusions and elongated or spherulated giant forms. Many mitochondria showed intimate association with rough surface endoplasmic reticulum (RER) adjacent to smooth surface endoplasmic reticulum (SER). GF-induced mitochondrial defects appear to represent nonspecific morphologic alterations similar to many reported in human hepatocytes in alcoholic liver disease, porphyria and various other toxic and metabolic liver diseases. They may reflect another common site of metabolic insult between two such diverse processes as GF-induced mouse porphyria and human alcoholic liver disease.
在给雄性瑞士韦伯斯特小鼠喂食含2.5%灰黄霉素（GF）的粉状饲料诱导卟啉症的过程中，观察了肝细胞线粒体的超微结构变化。在3天至22周之间发现了各种散在的线粒体改变。单独喂食粉状饲料的对照小鼠肝细胞中的线粒体看起来完好无损。7周后，改变的线粒体数量和显著程度增加，15周后尤为明显，同时伴有明显肝肿大和马洛里小体（MB）形成。在几个肝细胞的一些线粒体中观察到的一种或多种变化包括：肿胀、相关髓鞘小体、基质密度增加、嵴排列紊乱、嵴内肿胀、形状怪异、副结晶包涵体以及拉长或球状巨型形态。许多线粒体与靠近滑面内质网（SER）的粗面内质网（RER）紧密相连。GF诱导的线粒体缺陷似乎代表了非特异性形态学改变，类似于在酒精性肝病、卟啉症以及各种其他毒性和代谢性肝病的人类肝细胞中报道的许多改变。它们可能反映了GF诱导的小鼠卟啉症和人类酒精性肝病这两个不同过程之间另一个常见的代谢损伤部位。
