Camejo G, Lalaguna F, López F, Starosta R
Atherosclerosis. 1980 Mar;35(3):307-20. doi: 10.1016/0021-9150(80)90129-x.
The preparation of a proteoglycan (PG) from human aortic intima-media is described. The PG was obtained from intima-media homogenates by differential centrifugation, exclusion chromatography and preparative agarose electrophoresis. Crude or purified preparations of the proteoglycan are capable of forming specific insoluble complexes with LDL, purified or in serum. This product has been labelled lipoprotein-complexing proteoglycan (LCP-3). On agarose and cellulose acetate electrophoresis LCP-3 appears as a single band. However, its glycosaminoglycan (GAG) moiety shows a composition and chromatographic behaviour compatible with hybrid or mixed chains of chondroitin-6-so4, dermatan sulfate and heparin and/or heparan sulfate. The specificity of LCP-3 for LDL disappears when it is treated with testicular hyaluronidase or proteolytic enzymes. Ionic strength, pH, Ca++ and Mg++ modulate the amount of LDL insolubilized. The amino acid composition of the protein from LCP-3 is that of a basic protein(s), perhaps bound covalently through xylose--serine residues to the GAG's. The estimated molecular weight of LCP-3 is 1 to 5 x 10(6) daltons. The presence of LCP-3 to intima-media and its specificity for interacting with LDL at conditions near to physiological ones are suggestive of the role that this type of structure may play in the association of the atherogenic lipoproteins with components of the arterial intima-media.
本文描述了从人主动脉内膜-中膜制备蛋白聚糖（PG）的方法。通过差速离心、排阻色谱和制备性琼脂糖电泳从内膜-中膜匀浆中获得该PG。蛋白聚糖的粗制品或纯制品能够与纯化的或血清中的低密度脂蛋白（LDL）形成特定的不溶性复合物。该产物被标记为脂蛋白复合蛋白聚糖（LCP-3）。在琼脂糖和醋酸纤维素电泳中，LCP-3表现为单一的条带。然而，其糖胺聚糖（GAG）部分显示出与软骨素-6-硫酸酯、硫酸皮肤素和肝素和/或硫酸乙酰肝素的杂合链或混合链相符的组成和色谱行为。当用睾丸透明质酸酶或蛋白水解酶处理时，LCP-3对LDL的特异性消失。离子强度、pH值、Ca++和Mg++调节不溶性LDL的量。LCP-3中蛋白质的氨基酸组成是一种碱性蛋白质的组成，可能通过木糖-丝氨酸残基与GAG共价结合。LCP-3的估计分子量为1至5×10（6）道尔顿。LCP-3在内膜-中膜中的存在及其在接近生理条件下与LDL相互作用的特异性表明，这种结构类型可能在动脉粥样硬化脂蛋白与动脉内膜-中膜成分的结合中发挥作用。
