Deacon R, Lumb M, Perry J, Chanarin I, Minty B, Halsey M, Nunn J
Eur J Biochem. 1980 Mar;104(2):419-23. doi: 10.1111/j.1432-1033.1980.tb04443.x.
Exposure of rats to a 50% N2O/oxygen mixture led to a rapid loss of methionine synthase activity in both liver and brain. This enzyme has vitamin B12 as a cofactor. There was impaired conversion of deoxyuridine to deoxythymidine by bone marrow cells and this defect followed loss of methionine synthase activity. There was no homocystinuria. Withdrawal of N2O was followed by a relatively slow recovery of methionine synthase activity over four days. The inactivation of vitamin B12 by N2O promises to be a valuable tool in the study of vitamin B12 metabolism.
将大鼠暴露于50%的一氧化二氮/氧气混合气体中，会导致肝脏和大脑中的蛋氨酸合酶活性迅速丧失。这种酶以维生素B12作为辅因子。骨髓细胞将脱氧尿苷转化为脱氧胸苷的过程受到损害，且这种缺陷在蛋氨酸合酶活性丧失之后出现。没有高胱氨酸尿症。停止一氧化二氮暴露后，蛋氨酸合酶活性在四天内相对缓慢地恢复。一氧化二氮使维生素B12失活，这有望成为研究维生素B12代谢的一种有价值的工具。
