Nieminen A L, Kangas L, Anttila M, Hautoniemi L
J Chromatogr. 1980 Jan 11;181(1):11-16. doi: 10.1016/s0378-4347(00)81263-3.
Methenamine (hexamethylenetetramine), a urinary disinfectant, was determined in human plasma and urine by gas-liquid chromatography with a short (10 m) open-bore glass capillary column (split ratio 1:20) and nitrogen-selective detector. An almost quantitative recovery (92.1%) was achieved by simple dilution of water-containing samples (0.5 ml) with acetone (4.5 ml). After centrifugation an aliquot (2 microliter) of the supernatant was injected into the gas chromatograph. Selectivity and sensitivity of the nitrogen detector allowed the quantitation of unchanged methenamine in plasma and urine up to 24 h after a single therapeutic dose of 1 g. Reproducibility of the method was 7.6 and 2.1% (C.V.) in serum and urine, respectively. The time required for the analysis of one sample was approx. 2 min. Due to the simple extraction and short analysis time it was possible to analyze the samples concurrently with sample taking. Absorption of standard tablets and an enterosoluble preparation of methenamine hippurate was compared.
乌洛托品（六亚甲基四胺）是一种尿液消毒剂，采用短（10米）开口内径玻璃毛细管柱（分流比1:20）和气态氮选择性检测器，通过气液色谱法测定人血浆和尿液中的乌洛托品。通过用丙酮（4.5毫升）简单稀释含水样（0.5毫升），实现了几乎定量的回收率（92.1%）。离心后，将上清液的等分试样（2微升）注入气相色谱仪。氮检测器的选择性和灵敏度使得在单次治疗剂量1克后长达24小时内，能够对血浆和尿液中未变化的乌洛托品进行定量。该方法在血清和尿液中的重现性分别为7.6%和2.1%（变异系数）。分析一个样品所需的时间约为2分钟。由于提取简单且分析时间短，有可能在取样的同时对样品进行分析。比较了标准片剂和马尿酸乌洛托品肠溶制剂的吸收情况。
