Durschlag R P, Robinson J L
J Nutr. 1980 Apr;110(4):816-21. doi: 10.1093/jn/110.4.816.
The effects of orotic acid consumption on hepatic lipid and nucleotide metabolism were examined concurrently in the rat. When orotic acid was fed at 1.0%, fatty liver development was evident by day 7 but not by day 3, whereas a decreased purine/pyrimidine ratio of hepatic acid-soluble nucleotides was noted by day 3. As nucleotide changes preceded hepatic fat accumulation, they could be a contributing factor in the etiology of fatty liver. When fed for 10 days, rats did not accumulate hepatic fat on a diet containing 0.1%, but did on 0.5 and 1.0%; similarly, the purine/pyrimidine ratio was not significantly affected on 0.1% but was decreased on both 0.5 and 1.0% orotic acid. These results indicate that orotic acid alters both hepatic lipid and nucleotides when consumed by rats at a level above 0.1%. As measured in these studies, hepatomegaly did not always accompany hepatic steatosis and appeared to be a subsequent development. In addition, L-dihydroorotate was shown to be as effective as orotic acid in increasing hepatic weight and lipid whereas uridine was ineffective.
在大鼠中同时研究了摄入乳清酸对肝脏脂质和核苷酸代谢的影响。当以1.0%的比例喂食乳清酸时，第7天出现明显的脂肪肝，但第3天未出现，而在第3天就注意到肝脏酸溶性核苷酸的嘌呤/嘧啶比值降低。由于核苷酸变化先于肝脏脂肪积累，它们可能是脂肪肝病因中的一个促成因素。喂食10天时，大鼠在含0.1%乳清酸的饮食中不会积累肝脏脂肪，但在含0.5%和1.0%乳清酸的饮食中会积累；同样，0.1%乳清酸对嘌呤/嘧啶比值无显著影响，但0.5%和1.0%乳清酸均会使其降低。这些结果表明，当大鼠以高于0.1%的水平摄入乳清酸时，会改变肝脏脂质和核苷酸。在这些研究中测量发现，肝脏肿大并不总是伴随肝脂肪变性，似乎是随后出现的情况。此外，L-二氢乳清酸在增加肝脏重量和脂质方面与乳清酸一样有效，而尿苷则无效。
