Lewin L M, Nevo Z, Marcus Z, Gabsu A, Nebel L
Arch Androl. 1978;1(2):163-7. doi: 10.3109/01485017808988333.
Cauda epididymal sperm of mature guinea pigs were incubated (37 degrees, 5% CO2 in air). 10% of the total enzyme activity was released into the medium in 4 hr, 30% in 24 hr. Addition of lysolecithin resulted in rapid release of hyaluronidase. Vitamin C (0.54 mM), sodium fluoride (0.02 M), and cholesterol increased the rate of release whereas citrate (20 mM) diminished it. No effect upon hyaluronidase release was noted upon addition of KCN (10(-2)M), progesterone (250 microgram/ml), testosterone (500 microgram/ml), spermine (1.15 mg/ml), inositol (5.6 mM), or chloroquine phosphate (0.54 mM).
将成熟豚鼠的附睾尾精子进行孵育（37摄氏度，空气中含5%二氧化碳）。4小时内，总酶活性的10%释放到培养基中，24小时内释放30%。添加溶血卵磷脂会导致透明质酸酶快速释放。维生素C（0.54 mM）、氟化钠（0.02 M）和胆固醇可提高释放速率，而柠檬酸盐（20 mM）则降低释放速率。添加氰化钾（10⁻²M）、孕酮（250微克/毫升）、睾酮（500微克/毫升）、精胺（1.15毫克/毫升）、肌醇（5.6 mM）或磷酸氯喹（0.54 mM）对透明质酸酶的释放没有影响。
