Barrowcliffe T W
Thromb Haemost. 1980 Feb 29;42(5):1434-45.
The technique of crossed immunoelectrophoresis has been used to study the binding to purified antithrombin III (At III) of heparin and other mucopolysaccharides. The technique was unable to detect differences among samples of whole heparin from various manufacturers, but proved useful in studying the binding of heparin fractions; at the same molarities, low and high molecular weight heparin fractions displayed equal binding ability to At III. A semi-synthetic heparin analogue showed no evidence of binding to At III, but a sample of heparan sulphate did interact with At III at a concentration 3 times that of heparin. Samples of purified At III from four different manufacturers all displayed heterogeneity with respect to heparin binding. A proportion of the total At III did not bind to heparin and, in one sample, this non-binding material constituted about 40% of the total. An antiserum made against purifed At III contained antibodies with different cross-reactivities against heparin bound and non-heparin bound At III.
交叉免疫电泳技术已被用于研究肝素及其他粘多糖与纯化的抗凝血酶III（At III）的结合情况。该技术无法检测出不同厂家的全肝素样品之间的差异，但在研究肝素组分的结合方面证明是有用的；在相同摩尔浓度下，低分子量和高分子量肝素组分对At III的结合能力相同。一种半合成肝素类似物没有显示出与At III结合的迹象，但硫酸乙酰肝素样品在浓度为肝素3倍时确实能与At III相互作用。来自四个不同厂家的纯化At III样品在肝素结合方面均表现出异质性。总At III中有一部分不与肝素结合，在一个样品中，这种不结合物质约占总量的40%。针对纯化的At III制备的抗血清含有对结合肝素和未结合肝素的At III具有不同交叉反应性的抗体。
