Chan V, Chan T K
Haemostasis. 1979;8(6):373-89. doi: 10.1159/000214328.
Heparin antithrombin III binding was studied by crossed immunoelectrophoresis. In plasma and purified antithrombin III standard, multicomponent patterns were obtained with low concentrations of mucosal heparin. There is evidence that antithrombin III may bind more than one heparin molecule. At high heparin concentration (greater than 16 U/ml), single symmetrical peaks were obtained. Serum samples showed two antithrombin III peaks due to a decreased heparin binding of the slower peak (2.1-3.9 times), which was probably antithrombin III-activated procoagulant complexes. Heparin analogue (A 73025) also bound antithrombin III in vitro but the mobility of the peak was slower than with mucosal heparin and only a single peak was obtained in serum samples. Radioimmunoassay showed a decreased binding of antithrombin III antibody to heparin-antithrombin III complex. Venous occlusion to the forearm resulted in a slow second peak in the plasma. Heparin therapy gave rise to a double peak in the plasma antithrombin III profile and with continuous infusion, quantitative decreases were noted in all subjects studied, two of whom rethrombosed at the end of 7 days therapy.
采用交叉免疫电泳法研究肝素与抗凝血酶III的结合情况。在血浆和纯化的抗凝血酶III标准品中，低浓度的黏膜肝素可产生多组分图谱。有证据表明抗凝血酶III可能结合不止一个肝素分子。在高肝素浓度（大于16 U/ml）时，可获得单一的对称峰。血清样本显示出两个抗凝血酶III峰，原因是较慢峰的肝素结合减少（2.1 - 3.9倍），该较慢峰可能是抗凝血酶III激活的促凝复合物。肝素类似物（A 73025）在体外也能结合抗凝血酶III，但峰的迁移率比黏膜肝素慢，且在血清样本中仅获得一个峰。放射免疫分析显示抗凝血酶III抗体与肝素 - 抗凝血酶III复合物的结合减少。对前臂进行静脉闭塞会导致血浆中出现缓慢的第二个峰。肝素治疗使血浆抗凝血酶III图谱出现双峰，持续输注时，所有研究对象的抗凝血酶III均出现定量减少，其中两名患者在7天治疗结束时再次发生血栓形成。
