来自牛肋软骨的0.05M氯化镧可溶部分中的聚糖和糖蛋白。

Glycans and glycoproteins in 0.05M LaCl3-soluble fraction from bovine costal cartilage.

作者信息

Futami T, Ototani N, Nagatsuka Y, Yosizawa Z

出版信息

Tohoku J Exp Med. 1980 Jan;130(1):55-61. doi: 10.1620/tjem.130.55.

DOI:10.1620/tjem.130.55

Abstract

The supernatant fraction (Fr. S) obtained by ten-fold dilution of the 0.5 M LaCl3-extract of bovine costal cartilage was fractionated by DEAE-Sephadex A-25 column chromatography, followed by gel filtration on Sepharose 4B. The resulting five subfractions were characterized by electrophoresis on cellulose acetate membranes before and after digestion with pronase, alpha-amylase, and chondroitinase AC, together with analytical data. The results showed that Fr. S contained a large quantity of glycogen (Fr. 1), three sialoglycoproteins (Frs. 2, 3 and 4-1), hyaluronic acid (Fr. 4-1), a small molecular size proteoglycan consisting of chondroitin sulfates and keratan sulfate (Fr. 4-2), and small quantities of collagen-like protein. The components in the corresponding subfractions of Fr. S from non-calcified (NCC) and calcified (CC) portions of the costal cartilage were similar. It is indicated that consumption of glycogen is remarkable on calcification.

摘要

通过对牛肋软骨的0.5M氯化镧提取物进行十倍稀释得到的上清液部分（Fr. S），先经DEAE-葡聚糖A-25柱色谱分离，然后在Sepharose 4B上进行凝胶过滤。所得的五个亚组分通过在经链霉蛋白酶、α-淀粉酶和软骨素酶AC消化前后的醋酸纤维素膜电泳以及分析数据进行表征。结果表明，Fr. S含有大量糖原（Fr. 1）、三种唾液酸糖蛋白（Frs. 2、3和4-1）、透明质酸（Fr. 4-1）、一种由硫酸软骨素和硫酸角质素组成的小分子蛋白聚糖（Fr. 4-2）以及少量类胶原蛋白。来自肋软骨非钙化（NCC）和钙化（CC）部分的Fr. S相应亚组分中的成分相似。这表明在钙化过程中糖原消耗显著。