Alley M R, Wells P W, Smith W D, Gardiner A C
Vet Pathol. 1980 May;17(3):372-80. doi: 10.1177/030098588001700311.
IgM was the main immunoglobulin seen by immunofluorescence and immunoperoxidase techniques in the nasal and bronchial glands of lambs before suckling. After suckling IgG was found in tissues at all levels of the respiratory tract. Some IgA also was found after suckling but there was little evidence of transport into secretion. In adult sheep IgA -and IgG-containing cells were found in nearly equal numbers in the nasal mucosa but IgA cells exceeded by 2.5:1 in the bronchial mucosa and by 2:1 in the lung. IgA cells usually outnumbered IgM cells by more than 3:1 in all tissues. The numbers of all three classes of immunoglobulin-containing cells increased in sheep with chronic respiratory disease and, although the IgA;IgG ration was reduced, IgA remained the major locally secreted immunoglobulin. Cytoplasmic staining of epithelial cells for IgA and IgM was seen most frequently in proliferating areas which suggested that immature cells were engaged actively in immunoglobulin transport.
在哺乳前的羔羊鼻腺和支气管腺中，免疫荧光和免疫过氧化物酶技术检测到的主要免疫球蛋白是IgM。哺乳后，在呼吸道各级组织中均发现了IgG。哺乳后也发现了一些IgA，但几乎没有证据表明其转运至分泌物中。在成年绵羊中，鼻黏膜中含IgA和IgG的细胞数量几乎相等，但支气管黏膜中IgA细胞数量超出2.5:1，肺中超出2:1。在所有组织中，IgA细胞数量通常比IgM细胞数量多3倍以上。患有慢性呼吸道疾病的绵羊中，所有三类含免疫球蛋白细胞的数量均增加，尽管IgA与IgG的比例降低，但IgA仍然是主要的局部分泌免疫球蛋白。上皮细胞中IgA和IgM的细胞质染色最常见于增殖区域，这表明未成熟细胞积极参与免疫球蛋白的转运。
